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Luiz Boiteux Piazza, 1290 - Cachoeira do Bom Jesus, Florianópolis - SC, 88056-300, Brazil","infoId":"6439388625382512","pictureUrl":"https://uspic3.ok.com/logo/ineed.png","videos":null,"title":"Animal Cell Protein Expression Researcher - Biotech/Seara (JBS)","content":"**About JBS and the Biotech Innovation Center**\n\n \n\n**JBS** is a Brazilian multinational and one of the world's largest food companies. With over **70 years of history**, we operate in more than **20 countries** and have a team of **over 280,000 people** who share the purpose of **feeding the world with the best possible food**.\n\n \n\nAs part of our commitment to innovation and sustainability, we created the **Biotech Innovation Center** in Florianópolis (SC). It is the **first research center in Brazil focused on cultivated protein development**, a technology that enables the production of real meat from animal cells.\n\n \n\nAt Biotech, we combine science, technology, and a highly skilled team to transform the future of food. Here, we create solutions that respect the planet and expand the possibilities of how we nourish people.\n\n **Responsibilities and duties** \n\nWe are seeking a researcher with solid experience in molecular and animal cell biology to develop innovative strategies for expressing high-value-added and therapeutic recombinant proteins.\n\n \n\nThe professional will be responsible for identifying, planning, executing, optimizing, and interpreting alternative routes of biological synthesis using cellular systems such as CHO, HEK293, MDCK, Sf9, and in-house established cell lines. The focus will be on constructing and characterizing stable and/or transient cell lines, with direct application in strategic R&D projects in biotechnology.\n\n \n\nAmong the main challenges of the position are:\n\n* Developing and improving cellular platforms for efficient production of complex proteins;\n* Integrating technical knowledge to overcome bottlenecks in expression, yield, and functionality;\n* Contributing to the production of biomolecules such as structural proteins, growth factors, monoclonal antibodies, and other industrially relevant compounds.\n\n \n\nThe researcher will work in a highly multidisciplinary environment, collaborating with teams in molecular biology, cell culture, bioinformatics, purification, and protein analysis, aiming to integrate omics data and build robust expression pipelines.\n\n \n\nMain responsibilities of the position:\n\n* Plan and conduct experiments for transient and stable expression of recombinant proteins in animal cells, covering steps such as vectorization, transfection, clone selection, and characterization.\n* Develop, optimize, and scale-up cell culture processes, both adherent and suspension conditions, focusing on evaluating productivity, quality, and consistency of the final product.\n* Apply advanced genetic engineering tools, including CRISPR/Cas9, transposons, site-specific recombination, and viral vectors, to modify host cell lines and insert expression cassettes.\n* Perform protein expression analyses using techniques such as Western blot, ELISA, SDS-PAGE, flow cytometry, immunofluorescence, and other relevant methodologies.\n* Collaborate with multidisciplinary teams in purification and functional and analytical characterization of produced proteins, contributing to the development of robust and reliable assays.\n* Document and communicate results in a structured manner through electronic notebooks, technical reports, and internal presentations, ensuring traceability and alignment with project 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